Enrichment of Resequencing Targets for Analysis of Rare Variants

Presented by RainDance Technologies
11^th Annual Advances in Genome Biology and Technology Meeting

Despite notable success in the identification of common variants using genome-wide association studies, these approaches often lack the resolution to identify a large portion of the total variation believed to contribute to disease. With an increased interest in understanding the contribution of variants with a range of frequency and effect, high-resolution methods such as targeted resequencing provide the opportunity to assay both common and rare variants in large cohort studies. In order to confidently detect rare variants, it is critical to obtain as complete coverage as possible for regions of interest.

RainDance Technologies has commercialized a platform to enable the most complete coverage of resequencing targets by leveraging the benefits of single-molecule PCR in a format that easily integrates into next-generation sequencing workflows. To explain the benefits of this approach, RainDance will hold an AGBT workshop, entitled “Enrichment of Resequencing Targets for Analysis of Rare Variants.”

The Sequence Enrichment application utilizes our RainStorm™ technology to manipulate picoliter volume droplets to generate more than two million independent PCR reactions per sample in a simple and robust process. The workshop will describe the use of this novel method for enrichment of resequencing targets with high efficiency resulting in the highest-quality data for analysis of rare variants in both coding and non-coding regions of the genome. In addition, the workshop will feature a preview of new applications from RainDance that include targeted methylation analysis and ultra-deep resequencing of heterogeneous samples.

If you miss the presentation, please contact us.

For more information, contact Jeremy Lambert at 781-861-6300 or lambertj@raindancetech.com.